High-Speed, Cortex-Wide Volumetric Recording of Neuroactivity at Cellular Resolution Using Light Beads Microscopy

Optical Neurotechnology, Research
Our paper entitled “High-Speed, Cortex-Wide Volumetric Recording of Neuroactivity at Cellular Resolution using Light Beads Microscopy” has been published in Nature Methods. This work details our new method Light Beads Microscopy (LBM) which makes use of a column of “Light Beads” – individual beams which are distinguishable in time and focus to different depths in the sample (Fig. 1a)  – in order to record from the entire depth range of a given volume within the dead time between consecutive pulses from our excitation laser. By combining LBM with a commercial mesoscope, we can image mesoscale and volumetric fields of view (FOVs) at the same rate that a conventional mesoscope records a single plane. As a result, LBM gives optical access to the activity of cortex-wide volumes, allowing recording of up…
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