Master Position available

Master Thesis position at MFPL / University of Vienna

The higher-order structure of chromatin is poorly understood, but it has been proposed that a protein complex called the cohesin complex controls chromatin structure by contributing to the formation of chromatin loops.
In collaboration with the group of Jan-Michael Peters at the Research Institute of Molecular Pathology (IMP) we intend to investigate several proteins that regulate the interaction of cohesin with chromatin, including the cohesin-associated protein Wapl which is thought to release cohesin from DNA. The project involves the use of super-resolution microscopy to look at static and dynamic changes in chromatin structure upon gene regulation at specific gene loci.

Preferred Qualifications:

  • Experience in molecular biology techniques.
  • Cell culture.

Skills/techniques to acquire:

  • State of the art genome editing tools (TALENs, CRISPR/CAS9).
  • Live-cell imaging.
  • Use of image analysis software.

Send CV and if possible a letter of recommendation to:

Wide field microscopy image (top) and super-resolution reconstruction (bottom) of GFP-taged cohesin complexes subunit after Wapl depletion.
Wide field microscopy image (top) and super-resolution reconstruction (bottom)
of GFP-tagged cohesin complexes after Wapl depletion.

More information about research: