Master Thesis position at MFPL / University of Vienna
The higher-order structure of chromatin is poorly understood, but it has been proposed that a protein complex called the cohesin complex controls chromatin structure by contributing to the formation of chromatin loops.
In collaboration with the group of Jan-Michael Peters at the Research Institute of Molecular Pathology (IMP) we intend to investigate several proteins that regulate the interaction of cohesin with chromatin, including the cohesin-associated protein Wapl which is thought to release cohesin from DNA. The project involves the use of super-resolution microscopy to look at static and dynamic changes in chromatin structure upon gene regulation at specific gene loci.
Preferred Qualifications:
- Experience in molecular biology techniques.
- Cell culture.
Skills/techniques to acquire:
- State of the art genome editing tools (TALENs, CRISPR/CAS9).
- Live-cell imaging.
- Use of image analysis software.
Send CV and if possible a letter of recommendation to: david.cisneros@univie.ac.at
of GFP-tagged cohesin complexes after Wapl depletion.