Motors proteins of the conserved kinesin-14 family have important roles in mitotic spindle organization and chromosome segregation. Previous studies have indicated that kinesin-14 motors are non-processive enzymes, working in the context of multi-motor ensembles that collectively organize microtubule networks. Here we show that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor proteins Cik1 or Vik1. By analyzing the single-molecule properties of engineered motors we demonstrate that the non-catalytic domain has a key role in the motility mechanism by acting as a 'foothold' that allows Kar3 to bias translocation towards the minus end. This mechanism rivals the speed and run length of conventional motors, can support transport of the Ndc80 complex in vitro and is critical for Kar3 function in vivo. Our findings provide an example for…
Our recent paper "Optimizing and extending light-sculpting microscopy for fast functional imaging in neuroscience" on the improvement of our previously published imaging technique can be accessed via this Website. [caption id="attachment_1218" align="alignright" width="1020"] Experimental setup and various modalities of light sculpting microscopy.[/caption]
"The Economist" describes the open worm project on this website and mentions our recently published technique as a crucial step in this crowd-funded project. Read "The Economist" article. Read more about Open Worm. Read more about our Method on our page. Read the paper here.
Our recent paper on "Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy” was published today in Nature Methods. Using light-field deconvolution microscopy for functional biological imaging, we were able to simultaneous record the activity of the entire nervous system of C. elegans and showed whole brain imaging in zebrafish larvae at 20Hz volume rate. Click here for further information.
Our recent paper on "Brain-wide 3D imaging of neuronal activity Caenorhabditis elegans with sculpted light" was published today in Nature Methods. Here, we introduce wide-field temporal focusing (WF-TEFO), a two-photon technique, which is based on light-sculpting and enables recording the activity of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution. Click here to read the abstract or browse the research section on our homepage, to read more about the new technique which enables neurobiologists to acquire realtime 3D-videos of active neurons (see video below).
We are happy to announce, that David Cisneros - a shared Postdoc of our group and the lab of Jan-Michael Peters at the IMP Vienna - has contributed to a recent Nature publication which sheds light on the organization of chromosome structure and segregation: Wapl is an essential regulator of chromatin structure and chromosome segregation These findings reveal that the stability of cohesin–DNA interactions is an important determinant of chromatin structure, and indicate that cohesin has an architectural role in interphase chromosome territories.
Recently, a review paper entitled "Reshaping the optical dimension in optogenetics" by Alipasha and his collaborator, Valentina Emiliani from the CNRS in Paris, got published in Current Opinion in Neurobiology. In the paper, they discuss and compare the main new optical techniques that have become available in recent years in the field of optogenetics. The paper can be accessed by clicking here.
Vibrational excitons in ionophores: Experimental probes for quantum coherence-assisted ion transport and selectivity in ion channels Our recent paper by Ziad Ganim, Andre Tokmakoff and Alipasha Vaziri has been published in the New Journal of Physics. You can access the publication at this link.