Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz

Optical Neurotechnology, Research
Our paper entitled “Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” has been published in Nature Methods. We present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of neurons within volumes of ⌀4 × 0.2 mm, located at up to 350 µm depth in the mouse cortex, at 18 volumes per second (Video 1). Using our optical design and computational approach we show recording of ~10,000 neurons across multiple cortical areas in mice using workstation-grade computing resources. Video 1 | Animated perspective rendering of neuron positions and calcium activity recorded using MesoLF in mouse cortex. Field of view: ⌀4000 × 200 µm. Depth range: 0–200 µm. Recording frame rate: 18 Hz. Real-time recording duration: 405 s. Playback speed-up: 25×. Labelling construct: AAV9-TRE3-2xsomaGCaMP7f. Information…
Read More
“Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” published in Nature Methods

“Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” published in Nature Methods

News, Publications
Our article entitled “Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” has been published in Nature Methods. Information flow across mesoscale, i.e., multi-millimeter-sized regions of the mammalian cortex is a key feature of high-level cognition and is known to underlie complex behaviors. Yet, tracing this information flow in a volumetric fashion at a cellular resolution and high speed has remained challenging. This is primarily because most established neuronal activity imaging methods, such as two-photon microscopy, rely on time-consuming point-by-point scanning of an excitation beam focus to read out neuronal activity, as reported by the fluorescence rate of designer proteins known as genetically encoded calcium indicators (GECIs). We present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of…
Read More