Our article entitled “Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” has been published in Nature Methods.
Information flow across mesoscale, i.e., multi-millimeter-sized regions of the mammalian cortex is a key feature of high-level cognition and is known to underlie complex behaviors. Yet, tracing this information flow in a volumetric fashion at a cellular resolution and high speed has remained challenging. This is primarily because most established neuronal activity imaging methods, such as two-photon microscopy, rely on time-consuming point-by-point scanning of an excitation beam focus to read out neuronal activity, as reported by the fluorescence rate of designer proteins known as genetically encoded calcium indicators (GECIs).
We present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of neurons in parallel, within volumes of ⌀4 × 0.2 mm, located at up to 350 µm depth in the mouse cortex, at 18 volumes per second. Using our optical design and computational approach we show recording of ~10,000 neurons across multiple cortical areas in mice using workstation-grade computing resources.
Congratulations to the entire team!