“Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” published in Nature Methods

Our article entitled “Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” has been published in Nature Methods.

Information flow across mesoscale, i.e., multi-millimeter-sized regions of the mammalian cortex is a key feature of high-level cognition and is known to underlie complex behaviors. Yet, tracing this information flow in a volumetric fashion at a cellular resolution and high speed has remained challenging. This is primarily because most established neuronal activity imaging methods, such as two-photon microscopy, rely on time-consuming point-by-point scanning of an excitation beam focus to read out neuronal activity, as reported by the fluorescence rate of designer proteins known as genetically encoded calcium indicators (GECIs).

We present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of neurons in parallel, within volumes of ⌀4 × 0.2 mm, located at up to 350 µm depth in the mouse cortex, at 18 volumes per second. Using our optical design and computational approach we show recording of ~10,000 neurons across multiple cortical areas in mice using workstation-grade computing resources.

Please read the full article or a short summary.

Congratulations to the entire team!