Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz
Our paper entitled “Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz” has been published in Nature Methods. We present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of neurons within volumes of ⌀4 × 0.2 mm, located at up to 350 µm depth in the mouse cortex, at 18 volumes per second (Video 1). Using our optical design and computational approach we show recording of ~10,000 neurons across multiple cortical areas in mice using workstation-grade computing resources. Video 1 | Animated perspective rendering of neuron positions and calcium activity recorded using MesoLF in mouse cortex. Field of view: ⌀4000 × 200 µm. Depth range: 0–200 µm. Recording frame rate: 18 Hz. Real-time recording duration: 405 s. Playback speed-up: 25×. Labelling construct: AAV9-TRE3-2xsomaGCaMP7f. Information…
