Motors proteins of the conserved kinesin-14 family have important roles in mitotic spindle organization and chromosome segregation. Previous studies have indicated that kinesin-14 motors are non-processive enzymes, working in the context of multi-motor ensembles that collectively organize microtubule networks. Here we show that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor proteins Cik1 or Vik1. By analyzing the single-molecule properties of engineered motors we demonstrate that the non-catalytic domain has a key role in the motility mechanism by acting as a 'foothold' that allows Kar3 to bias translocation towards the minus end. This mechanism rivals the speed and run length of conventional motors, can support transport of the Ndc80 complex in vitro and is critical for Kar3 function in vivo. Our findings provide an example for…
Our recent paper "Optimizing and extending light-sculpting microscopy for fast functional imaging in neuroscience" on the improvement of our previously published imaging technique can be accessed via this Website. [caption id="attachment_1218" align="alignright" width="1020"] Experimental setup and various modalities of light sculpting microscopy.[/caption]
The Vaziri lab has successfully secured with colleagues at IMP and IMBA two grants in the WWTF's Life Sciences Call 2014 - "IMAGING Innovative Biological and Biomedical Applications of Novel Imaging Technologies". The projects will be dedicated to building new super-resolution and high-speed whole-brain functional imaging techniques in behaving animals. Congratulations!
Friederike Schlumm and Christoph Götz from the Vaziri lab won together with Isabel Grießhammer (IMBA) the VBC Art&Science Contest with their video: "Incubate over night" https://www.youtube.com/watch?feature=player_embedded&v=lVchRXwjJRs Incubation is a widely used method in research. Nevertheless, the underlying mechanisms are not well understood. In this project we aimed to visualize this crucial process. We therefore applied the imaging technique "stop-motion" to study an overnight incubation. Our results reveal new insights into scientific experiments. Four teams participated, with four terrific art pieces, which were exhibited in our Cafeteria, and the VBCers were voting for the best piece. See the other art pieces at: http://www.vbcphdprogramme.at/current-students/art-science-contest/
http://vimeo.com/96490051 Assoc. Prof. Dr. Alipasha Vaziri was interviewed by the WWTF about his motivations to do science in Vienna and gives a general overview over the research interests our group. Find also other interesting Interviews and learn more about the WWTF on their Homepage.
"The Economist" describes the open worm project on this website and mentions our recently published technique as a crucial step in this crowd-funded project. Read "The Economist" article. Read more about Open Worm. Read more about our Method on our page. Read the paper here.
Our recent paper on "Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy” was published today in Nature Methods. Using light-field deconvolution microscopy for functional biological imaging, we were able to simultaneous record the activity of the entire nervous system of C. elegans and showed whole brain imaging in zebrafish larvae at 20Hz volume rate. Click here for further information.