Our paper entitled “High-speed volumetric imaging of neuronal activity in freely moving rodents” has been published in Nature Methods.
We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm at 16 Hz frame rate in the hippocampus of freely moving mice. We demonstrate that neurons as close as ~15 µm to each other and at depths up to 360 µm can be discriminated. MiniLFM is based on Light Field Microscopy (LFM), a 3D imaging technique that our group has established in a previous publication as a versatile neural recording technique, and the Seeded Iterative Demixing (SID) algorithm, which we presented recently, and which extends LFM into more strongly scattering tissue such as the mammalian cortex.
Please also have a look at the press release of the Rockefeller University.