“High-Speed, Cortex-Wide Volumetric Recording of Neuroactivity at Cellular Resolution Using Light Beads Microscopy” published in Nature Methods
We are excited to share that our paper entitled “High-Speed, Cortex-Wide Volumetric Recording of Neuroactivity at Cellular Resolution using Light Beads Microscopy” has been published in Nature Methods. Two-photon microscopy has enabled high-resolution imaging of neuroactivity at depth within scattering brain tissue. However, its various realizations have not overcome the tradeoffs between speed and spatiotemporal sampling that would be necessary to enable mesoscale volumetric recording of neuroactivity at cellular resolution and speed compatible with resolving calcium transients. This work details our new method Light Beads Microscopy (LBM), which makes use of a column of “Light Beads” – individual beams which are distinguishable in time and focus to different depths in the sample – in order to record from the entire depth range of a given volume within the dead time between…