“Neuronal basis of decision making at the single trial level” published in Cell

“Neuronal basis of decision making at the single trial level” published in Cell

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Our paper entitled “Cerebellar neurodynamics predict decision timing and outcome on single-trial level” has been published in Cell. In this work we have investigated the neuronal basis of motor planning and decision making at single-trial level, at single-cell resolution and on the level of the whole brain. To study this, we have combined high-speed volumetric calcium imaging based on light-field microscopy (LFM) previously developed in our lab with a new learning paradigm in larval zebrafish for operant conditioning, Relief Of Aversive Stimulus by Turn (ROAST). We have demonstrated that that decision making relies on integration and coordination of information across different brain regions. Within the brain-wide network, we discovered a particularly strong preparatory activity in the cerebellum which allowed us to quantitatively predict both, the time point and the outcome of the…
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“HyMS  microscopy” published in Cell

“HyMS microscopy” published in Cell

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Our paper entitled “Volumetric Ca2+ Imaging in the Mouse Brain using Hybrid Multiplexed Sculpted Light (HyMS) Microscopy” has been published in Cell. We have developed a modular platform named Hybrid Multiplexed Sculpted Light (HyMS) microscopy featuring a systems-wide design paradigm that maximizes the acquisition volume size and speed while maintaining fidelity for obtaining single neuron activity traces. Our modular design utilizes a hybrid two- and three-photon acquisition and allows for volumetric recording of neuroactivity at single-cell resolution within up to 1 × 1 × 1.22 mm volumes at up to 17 Hz in awake behaving mice. We establish the capabilities and potential of the different configurations of HyMS microscopy at depth and across brain regions by applying it to in-vivo recording of up to 12,000 neurons in mouse auditory cortex,…
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