Our recent paper on “Brain-wide 3D imaging of neuronal activity Caenorhabditis elegans with sculpted light” was published today in Nature Methods.
Here, we introduce wide-field temporal focusing (WF-TEFO), a two-photon technique, which is based on light-sculpting and enables recording the activity of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution.
Click here to read the abstract or browse the research section on our homepage, to read more about the new technique which enables neurobiologists to acquire realtime 3D-videos of active neurons (see video below).