We are excited to announce the publication of our article, “A versatile platform for two-photon neuronal population voltage imaging across cortical depths,” in Nature Methods, a collaborative effort between the St-Pierre Lab at BCM and the Vaziri Lab at The Rockefeller University, led by Dr. Jingkun Guo.
In this work, we demonstrate the realization of a new Flexible lateral-temporal Multiplexing (FlatMux) optical cavity for a scalable large-scale, deep, multi-plane and efficient two-photon neuronal populational voltage imaging in the scattering mouse brain.
We showcase FlatMux’s versatility and flexibility including a large field-of-view (590 × 400 μm2) mode, a high-speed mode with a frame rate of 2,000 Hz, a deep-tissue imaging mode that records cortical spiking activity at up to 500-µm depth, a dual-plane imaging mode allowing simultaneous recording of population spiking activity of neurons within cortical L2/3 and L4, and high-SNR imaging mode that records subthreshold neuronal activity and high-SNR spiking activity while minimizing pixel crosstalk and bleaching.
Read our full publication here.
Congratulations to the entire team!