New publication in Nature Methods

New publication in Nature Methods

News, Publications
Our paper entitled “Video rate volumetric Ca2+ imaging across cortical layers using Seeded Iterative Demixing (SID) microscopy” has been published in Nature Methods. We present a novel computational technique termed Seeded Iterative Demixing (SID) that allows for capturing neuronal dynamics in vivo within a volume of 900 × 900 × 260 µm, located as deep as 380 µm in the mouse cortex and hippocampus, and at a very high volume rate of 30 Hz. SID is based on Light Field Microscopy (LFM), a 3D imaging technique that our group has established in a previous publication as a versatile neural recording technique for weakly scattering specimen, such as larval zebrafish. SID extends LFM into more strongly scattering tissue such as the mammalian cortex by seeding a machine learning algorithm with remaining…
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