Our paper entitled “Fast volumetric calcium imaging across multiple cortical layers using sculpted light” has been published in Nature Methods.
In this work, we present a novel method based on light sculpting that enables unbiased single and dual-plane high-speed (up to 160 Hz) calcium imaging, as well as in vivo volumetric calcium imaging of a mouse cortical column (500x500x500 µm) at single-cell resolution and fast volume rates (3 – 6 Hz). This is achieved by tailoring the point-spread function of our microscope to the structures of interest while maximizing the signal-to-noise ratio while using a home-built fiber laser amplifier with pulses that are synchronized to the imaging voxel speed. Together, these innovations have enabled the near-simultaneous in-vivo recording of calcium dynamics of several thousand active neurons across cortical layers and in the hippocampus of awake behaving mice.
Read the publication or a short summary.
Please also have a look at the press release of the Rockefeller University.
We congratulate her on the PhD title awarded by the university of Singapore. Previously, she worked on neurobehavioral manifestations of decision making. Recently, Qian Lin joined our group to strengthen our research focus on whole brain imaging to unravel the collective function of brain structures in simple behavioral paradigms.
We welcome Jeroen Delcour (Netherlands) and Ren Zicheng (China) as visiting scientists in our group. They will try to understand specific brain functions by applying our previously developed whole-brain imaging techniques (see our Publications) to animal models.
We are happy to announce that Ass.-Prof. Dr. Alipasha Vaziri was awarded the Förderungspreis 2014 of the City of Vienna.
The City of Vienna recognizes outstanding artists and scientists with honors in the form of prizes and scholarships. These have been awarded since 1951 in the fields of music (composition), literature, visual arts and architecture, science and popular education.
Read more about it here (german).